Incubation:Article Title: Cardio-renal effect of dapagliflozin and dapagliflozin- saxagliptin combination on CD34 + ve hematopoietic stem cells (HSCs) and podocyte specific markers in type 2 diabetes (T2DM) subjects: a randomized trial.
Article Snippet: Extracellular vesicle extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer’s protocols (Bio-Rad, USA). .. After incubation with 5% nonfat milk in TBST (Bio-Rad, USA) (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min. .. The membrane was washed once with TBST and incubated with antibodies against CD9 (1:1000), CD81 (1:1000), CD63 (1:1000), HSP70 (1:1000) (Sysetem Biosciences, USA), anti-podocalyxin (PODXL, 1:1000), anti-wilms tumor protein (1:1000) and anti-Nephrin antibody (1:1000) at 4 °C for 12 h (Abcam, USA).
Article Title: Cardio-renal Effect of Dapagliflozin AND Dapagliflozin- Saxagliptin combination on CD34+ve Hematopoietic Stem Cells (HSCs) and Podocyte specific markers in Type 2 Diabetes (T2DM) subjects: a randomized trial.
Article Snippet: Extracellular vesicle extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene di uoride membrane using a transfer apparatus according to the manufacturer’s protocols (Bio-Rad, USA). .. After incubation with 5% nonfat milk in TBST (Bio-Rad, USA) (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min. .. The membrane was washed once with TBST and incubated with antibodies against CD9 (1:1000), CD81 (1:1000), CD63 (1:1000), HSP70 (1:1000) (Sysetem Biosciences, USA), anti-podocalyxin (PODXL, 1:1000), anti-wilms tumor protein (1:1000) and anti-Nephrin antibody (1:1000) at 4°C for 12 h (Abcam, USA).
Article Title: Cardio-renal effect of dapagliflozin and dapagliflozin- saxagliptin combination on CD34 + ve hematopoietic stem cells (HSCs) and podocyte specific markers in type 2 diabetes (T2DM) subjects: a randomized trial
Article Snippet: Extracellular vesicle extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer’s protocols (Bio-Rad, USA). .. After incubation with 5% nonfat milk in TBST (Bio-Rad, USA) (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min. .. The membrane was washed once with TBST and incubated with antibodies against CD9 (1:1000), CD81 (1:1000), CD63 (1:1000), HSP70 (1:1000) (Sysetem Biosciences, USA), anti-podocalyxin (PODXL, 1:1000), anti-wilms tumor protein (1:1000) and anti-Nephrin antibody (1:1000) at 4 °C for 12 h (Abcam, USA).
Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 2-3) or IRDye (FIG. 4, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 2-3) or scanned on an Odyssey CLx Imaging system (FIG. 4). ..
Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 minutes, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 2-3) or IRDye (FIG. 4, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 2-3) or scanned on an Odyssey CLx Imaging system (FIG. 4). ..
Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 1-2) or IRDye (FIG. 3, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 1-2) or scanned on an Odyssey CLx Imaging system (FIG. 3). ..
Article Title: Genetically engineered Magnesium/Manganese nanoparticles for cancer radioimmunotherapy
Article Snippet: The different treated B16F10 cells and BMDCs were lysed in RIPA buffer (Beyotime) which containing the protease- and phosphatase-inhibitors (Roche) for inhibiting degradation. .. After measured the concentration of solution with the BCA protein assay kit, the lysates were denatured and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Then, transferred the proteins to PVDF membranes (Biorad), and blocked the membranes with 5% nonfat milk in TBST (Tris-buffered Saline and 0.1% Tween 20) for 1 h at room temperature, and sequentially incubated it with the primary antibodies at 4 °C overnight. .. The membranes were subsequently incubated with horseradish peroxidase (HRP)-linked secondary antibody (Cell Signaling, Beverly, MA) for 2 h after washed with 1× TBST for three times.
Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 minutes, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 2-3) or IRDye (FIG. 4, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 2-3) or scanned on an Odyssey CLx Imaging system (FIG. 4). ..
Membrane:Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 2-3) or IRDye (FIG. 4, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 2-3) or scanned on an Odyssey CLx Imaging system (FIG. 4). ..
Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 minutes, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 2-3) or IRDye (FIG. 4, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 2-3) or scanned on an Odyssey CLx Imaging system (FIG. 4). ..
Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 1-2) or IRDye (FIG. 3, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 1-2) or scanned on an Odyssey CLx Imaging system (FIG. 3). ..
Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 minutes, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 2-3) or IRDye (FIG. 4, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 2-3) or scanned on an Odyssey CLx Imaging system (FIG. 4). ..
Imaging:Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 2-3) or IRDye (FIG. 4, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 2-3) or scanned on an Odyssey CLx Imaging system (FIG. 4). ..
Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 minutes, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 2-3) or IRDye (FIG. 4, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 2-3) or scanned on an Odyssey CLx Imaging system (FIG. 4). ..
Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 min, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 1-2) or IRDye (FIG. 3, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 1-2) or scanned on an Odyssey CLx Imaging system (FIG. 3). ..
Article Title: Compounds and uses thereof
Article Snippet: Whole cell extracts were fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane using a transfer apparatus according to the manufacturer's protocols (Bio-Rad). .. After incubation with 5% nonfat milk in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 60 minutes, the membrane was incubated with antibodies against BRD9 (1:1,000, Bethyl laboratory A303-781A), GAPDH (1:5,000, Cell Signaling Technology), and/or MBP (1:1,000, BioRad) overnight at 4° C. Membranes were washed three times for 10 min and incubated with anti-mouse or anti-rabbit antibodies conjugated with either horseradish peroxidase (HRP, FIGS. 2-3) or IRDye (FIG. 4, 1:20,000, LI-COR) for at least 1 h. Blots were washed with TBST three times and developed with either the ECL system according to the manufacturer's protocols (FIGS. 2-3) or scanned on an Odyssey CLx Imaging system (FIG. 4). ..
Concentration Assay:Article Title: Genetically engineered Magnesium/Manganese nanoparticles for cancer radioimmunotherapy
Article Snippet: The different treated B16F10 cells and BMDCs were lysed in RIPA buffer (Beyotime) which containing the protease- and phosphatase-inhibitors (Roche) for inhibiting degradation. .. After measured the concentration of solution with the BCA protein assay kit, the lysates were denatured and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Then, transferred the proteins to PVDF membranes (Biorad), and blocked the membranes with 5% nonfat milk in TBST (Tris-buffered Saline and 0.1% Tween 20) for 1 h at room temperature, and sequentially incubated it with the primary antibodies at 4 °C overnight. .. The membranes were subsequently incubated with horseradish peroxidase (HRP)-linked secondary antibody (Cell Signaling, Beverly, MA) for 2 h after washed with 1× TBST for three times.
BIA-KA:Article Title: Genetically engineered Magnesium/Manganese nanoparticles for cancer radioimmunotherapy
Article Snippet: The different treated B16F10 cells and BMDCs were lysed in RIPA buffer (Beyotime) which containing the protease- and phosphatase-inhibitors (Roche) for inhibiting degradation. .. After measured the concentration of solution with the BCA protein assay kit, the lysates were denatured and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Then, transferred the proteins to PVDF membranes (Biorad), and blocked the membranes with 5% nonfat milk in TBST (Tris-buffered Saline and 0.1% Tween 20) for 1 h at room temperature, and sequentially incubated it with the primary antibodies at 4 °C overnight. .. The membranes were subsequently incubated with horseradish peroxidase (HRP)-linked secondary antibody (Cell Signaling, Beverly, MA) for 2 h after washed with 1× TBST for three times.
Gel Electrophoresis:Article Title: Genetically engineered Magnesium/Manganese nanoparticles for cancer radioimmunotherapy
Article Snippet: The different treated B16F10 cells and BMDCs were lysed in RIPA buffer (Beyotime) which containing the protease- and phosphatase-inhibitors (Roche) for inhibiting degradation. .. After measured the concentration of solution with the BCA protein assay kit, the lysates were denatured and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Then, transferred the proteins to PVDF membranes (Biorad), and blocked the membranes with 5% nonfat milk in TBST (Tris-buffered Saline and 0.1% Tween 20) for 1 h at room temperature, and sequentially incubated it with the primary antibodies at 4 °C overnight. .. The membranes were subsequently incubated with horseradish peroxidase (HRP)-linked secondary antibody (Cell Signaling, Beverly, MA) for 2 h after washed with 1× TBST for three times.
Saline:Article Title: Genetically engineered Magnesium/Manganese nanoparticles for cancer radioimmunotherapy
Article Snippet: The different treated B16F10 cells and BMDCs were lysed in RIPA buffer (Beyotime) which containing the protease- and phosphatase-inhibitors (Roche) for inhibiting degradation. .. After measured the concentration of solution with the BCA protein assay kit, the lysates were denatured and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Then, transferred the proteins to PVDF membranes (Biorad), and blocked the membranes with 5% nonfat milk in TBST (Tris-buffered Saline and 0.1% Tween 20) for 1 h at room temperature, and sequentially incubated it with the primary antibodies at 4 °C overnight. .. The membranes were subsequently incubated with horseradish peroxidase (HRP)-linked secondary antibody (Cell Signaling, Beverly, MA) for 2 h after washed with 1× TBST for three times.
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